Câu 20: USMLE: United States Medical Licensing Examination

A technician wants to determine whether cytomegalovirus (CMV) DNA is present in the blood of a bone marrow transplant recipient. DNA purified from the leukocytes of the patient is reacted in a mixture containing oligonucleotides specific for CMV DNA, thermostable DNA polymerase, and nucleotides. Repetitive cycles of heating and cooling are performed, and the reaction product is detected by gel electrophoresis. The technician most likely used which of the following laboratory procedures on this patient's blood?

1. A. Northern blotting
2. B. Polymerase chain reaction — đáp án hiện tại
3. C. Reverse transcription
4. D. Southern blotting
5. E. Western blotting